(S)-canadine synthase

In enzymology, a (S)-canadine synthase is an enzyme that catalyzes the chemical reaction


 * (S)-tetrahydrocolumbamine + NADPH + H$+$ + O$2$ $$\rightleftharpoons$$ (S)-canadine + NADP$+$ + 2 H$2$O

The 4 substrates of this enzyme are (S)-tetrahydrocolumbamine, NADPH, H$+$, and O$2$, whereas its 3 products are Canadine, NADP$+$, and H$2$O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O$2$ as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O$2$ with NADH or NADPH as one donor, and the other dehydrogenated. The systematic name of this enzyme class is (S)-tetrahydrocolumbamine,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming). Other names in common use include (S)-tetrahydroberberine synthase, and (S)-tetrahydrocolumbamine oxidase (methylenedioxy-bridge-forming). This enzyme participates in alkaloid biosynthesis i. It employs one cofactor, heme-thiolate(P-450).