1916 Cal Poly Mustangs football team

The 1916 Cal Poly Mustangs football team represented California Polytechnic School, now California Polytechnic State University, in the 1916 college football season. Led by second-year head coach D. W. Schlosser, Cal Poly compiled a record of 1–2 and were outscored by their opponents 56 to 25.

Cal Poly was a two-year school until 1941.