1927 Cal Poly Mustangs football team

The 1927 Cal Poly Mustangs football team represented California Polytechnic School—now known as California Polytechnic State University, San Luis Obispo—as a member of the California Coast Conference (CCC) during the 1927 college football season. Led by seventh-year head coach Al Agosti, Cal Poly compiled an overall record of 2–4–1 with a mark of 1–3 in conference play. The team was outscored by its opponents 93 to 46 for the season. The Mustangs played home games in San Luis Obispo, California.

Cal Poly was a two-year school until 1941.