1959–60 Eerste Divisie

The Dutch Eerste Divisie in the 1959–60 season was contested by 33 teams, divided in one group of sixteen and one group of seventeen teams. GVAV and Alkmaar '54 won the championship.

Eerste Divisie A
Promoted from the 1958–59 Tweede Divisie: Relegated from the 1958–59 Eredivisie: Entered from the B-group:
 * 't Gooi
 * Veendam
 * TSV NOAD
 * SBV Excelsior
 * GVAV
 * HVC
 * KFC
 * Rigtersbleek
 * Stormvogels
 * Vitesse Arnhem
 * De Volewijckers

Eerste Divisie B
Promoted from the 1958–59 Tweede Divisie: Relegated from the 1958–59 Eredivisie: Entered from the A-group:
 * DHC
 * Go Ahead
 * SHS (relegated as Holland Sport)
 * AGOVV Apeldoorn
 * Alkmaar '54
 * DFC
 * FC Eindhoven
 * De Graafschap
 * Leeuwarden
 * Limburgia
 * VSV
 * ZFC