1959 Cal Poly Mustangs football team

The 1959 Cal Poly Mustangs football team represented California Polytechnic State College—now known as California Polytechnic State University, San Luis Obispo—as a member of the California Collegiate Athletic Association (CCAA) during the 1959 NCAA College Division football season. Led by tenth-year head coach LeRoy Hughes, Cal Poly compiled an overall record of 6–3 with a mark of 3–2 in conference play, tying for second place in the CCAA. The Mustangs played home games at Mustang Stadium in San Luis Obispo, California.