1960–61 Tweede Divisie

The Dutch Tweede Divisie in the 1960–61 season was contested by 18 teams, that would for the first time play in one league instead of two.

Two teams would be directly promoted to the Eerste Divisie. The 3rd- and 4th-place finishers would play against one another and the winner would take on a team from the Eerste Divisie itself.

New entrants
Relegated from the Eerste Divisie
 * Rigtersbleek
 * De Graafschap

Promotion/relegation play-off
RKVV Wilhelmina were promoted to the Eerste Divisie.