1964–65 Eerste Divisie

The Dutch Eerste Divisie in the 1964–65 season was contested by 16 teams. Willem II won the championship for the second time.

New entrants
Promoted from the 1963–64 Tweede Divisie: Relegated from the 1963–64 Eredivisie:
 * Alkmaar '54
 * NEC
 * Blauw-Wit Amsterdam
 * FC Volendam

Championship match
Willem II & Elinkwijk played a match to determine the league champions (on the field of BVV).