1965–66 Tweede Divisie

The Dutch Tweede Divisie in the 1965–66 season was contested by 30 teams, divided in two groups. It would be the last season the Tweede Divisie would be divided in two. From next season onwards, all teams would participate in one league. This also meant that more teams than before were promoted to the Eerste Divisie. No teams had to relegate to amateur football.

Group A
Relegated from the Eerste Divisie: Entered from the B-group:
 * Veendam
 * HVC
 * SC Gooiland (last season playing as 't Gooi)

Group B
Relegated from the Eerste Divisie: Entered from the A-group: FC Den Bosch played as BVV last season
 * Excelsior
 * RCH
 * HFC Haarlem
 * HFC EDO