1965 Appalachian State Mountaineers football team

The 1965 Appalachian State Mountaineers football team was an American football team that represented Appalachian State Teachers College (now known as Appalachian State University) as a member of the Carolinas Conference during the 1965 NAIA football season. In their first year under head coach Carl Messere, the Mountaineers compiled an overall record of 5–5, with a mark of 3–3 in conference play, and finished tied for fourth in the Carolinas Conference.