1965 NCAA University Division baseball tournament

The 1965 NCAA University Division baseball tournament was played at the end of the 1965 NCAA University Division baseball season to determine the national champion of college baseball. The tournament concluded with eight teams competing in the College World Series, a double-elimination tournament in its nineteenth year. Eight regional districts sent representatives to the College World Series with preliminary rounds within each district serving to determine each representative. These events would later become known as regionals. Each district had its own format for selecting teams, resulting in 23 teams participating in the tournament at the conclusion of their regular season, and in some cases, after a conference tournament. The nineteenth tournament's champion was Arizona State, coached by Bobby Winkles. The Most Outstanding Player was Sal Bando of Arizona State.

Regionals
The opening rounds of the tournament were played across seven district sites across the country, each consisting of a field of two to four teams. Each district tournament, except District 2, was double-elimination. The winners of each district advanced to the College World Series.

Bold indicates winner. * indicates extra innings.

District 6
Texas automatically qualified for the College World Series out of District 6.

All-Tournament Team
The following players were members of the All-Tournament Team.

Notable players

 * Arizona State: John Pavlik, Sal Bando, Duffy Dyer, Rick Monday, Al Schmelz
 * Connecticut:
 * Florida State: Randy Brown, Jim Lyttle
 * Lafayette:
 * Ohio State: Steve Arlin, Chuck Brinkman, Russ Nagelson
 * St. Louis:
 * Texas: Joe Hague, Gary Moore
 * Washington State: Danny Frisella

Tournament notes

 * Ohio State's 15 inning victory over Washington State is the longest game in College World Series history