1965 Prairie View A&M Panthers football team

The 1965 Prairie View A&M Panthers football team represented Prairie View A&M College of Texas (now known as Prairie View A&M University) as a member of the Southwestern Athletic Conference (SWAC) during the 1965 NCAA College Division football season. Led by 17th-year head coach Billy Nicks, the Panthers compiled an overall record of 5–3–1, with a conference record of 3–3–1, and finished tied for fourth in the SWAC.