1990 Eastern Kentucky Colonels football team

The 1990 Eastern Kentucky Colonels football team represented Eastern Kentucky University as a member of the Ohio Valley Conference (OVC) during the 1990 NCAA Division I-AA football season. Led by 27th-year head coach Roy Kidd, the Colonels compiled an overall record of 10–2, with a mark of 5–1 in conference play, and finished as OVC co-champion. Eastern Kentucky advanced to the NCAA Division I-AA First Round and were defeated by Furman.