1990 Italian Open – Women's singles

Monica Seles defeated Martina Navratilova in the final, 6–1, 6–1 to win the women's singles tennis title at the 1990 Italian Open.

Gabriela Sabatini was the two-time defending champion, but lost in the semifinals to Navratilova.

Seeds
The first eight seeds received a bye to the second round. • # 🇺🇸 Martina Navratilova (final)

• # Monica Seles (champion)

• # 🇪🇸 Arantxa Sánchez Vicario (third round)

• # 🇦🇷 Gabriela Sabatini (semifinals)

• # Manuela Maleeva (quarterfinals)

• # 🇪🇸 Conchita Martínez (quarterfinals)

• # 🇫🇷 Nathalie Tauziat (second round)

• # 🇦🇹 Judith Wiesner (second round)

• # 🇵🇪 Laura Gildemeister (second round)

• # 🇮🇹 Raffaella Reggi (third round)

• # 🇨🇦 Helen Kelesi (semifinals)

• # 🇺🇸 Jennifer Capriati (quarterfinals)

• # 🇸🇪 Catarina Lindqvist (quarterfinals)

• # 🇮🇹 Sandra Cecchini (third round)

• # Leila Meskhi (second round)

• # Cathy Caverzasio (third round)