1991 Italian Open – Women's singles

Gabriela Sabatini defeated the defending champion Monica Seles in the final, 6–3, 6–2 to win the women's singles tennis title at the 1991 Italian Open.

Seeds
The top eight seeds received a bye to the second round. • # Monica Seles (final)

• # 🇦🇷 Gabriela Sabatini (champion)

• # 🇺🇸 Martina Navratilova (quarterfinal)

• # 🇺🇸 Mary Joe Fernández (semifinal)

• # 🇪🇸 Conchita Martínez (semifinal)

• # Manuela Maleeva-Fragniere (third round)

• # 🇺🇸 Jennifer Capriati (quarterfinal)

• # Leila Meskhi (quarterfinal)

• # 🇫🇷 Nathalie Tauziat (third round)

• # n/a

• # 🇮🇹 Sandra Cecchini (first round)

• # 🇨🇦 Helen Kelesi (third round)

• # 🇩🇪 Anke Huber (third round)

• # 🇦🇷 Mercedes Paz (first round)

• # 🇧🇬 Magdalena Maleeva (first round)

• # 🇮🇹 Federica Bonsignori (first round)