1994 Italian Open – Women's singles

Defending champion Conchita Martínez defeated Martina Navratilova in the final, 7–6(7–5), 6–4 to win the women's singles tennis title at the 1994 Italian Open.

Seeds
The first eight seeds received a bye to the second round. • # 🇪🇸 Conchita Martínez (champion)

• # 🇺🇸 Martina Navratilova (final)

• # 🇦🇷 Gabriela Sabatini (second round)

• # 🇩🇪 Anke Huber (second round)

• # 🇫🇷 Mary Pierce (third round)

• # Amanda Coetzer (second round)

• # 🇯🇵 Naoko Sawamatsu (quarterfinals)

• # 🇫🇷 Nathalie Tauziat (quarterfinals)

• # 🇦🇹 Judith Wiesner (quarterfinals)

• # Brenda Schultz (third round)

• # Leila Meskhi (withdrew)

• # 🇮🇹 Linda Ferrando (first round)

• # 🇺🇸 Ann Grossman (third round)

• # 🇺🇸 Sandra Cacic (second round)

• # 🇩🇪 Meike Babel (first round)

• # Miriam Oremans (first round)