1999–2000 Danish 1st Division

The 1999–2000 Danish 1st Division season was the 55th season of the Danish 1st Division league championship and the 14th consecutive as a second tier competition governed by the Danish Football Association.

The division-champion and runner-up promoted to the 2000–01 Danish Superliga. The teams in the 13th to 16th spots relegated to the 2000–01 Danish 2nd Division.