1999–2000 Eerste Divisie

The Dutch Eerste Divisie in the 1999–00 season was contested by 18 teams. NAC Breda won the championship.

New entrants
Relegated from the 1998–99 Eredivisie
 * NAC Breda

Promotion/relegation play-offs
In the promotion/relegation competition, eight entrants (six from this league and two from the Eredivisie) entered in two groups. The group winners were promoted to the Eredivisie.