1999–2000 National First Division

The 1999–2000 National First Division was the 4th season of the second tier of South African soccer since the reorganisation in 1996.

It consisted of two streams, an Inland stream of 16 teams, and a Coastal stream of 14 teams.

The Coastal stream was won by Golden Arrows, the third consecutive team from Durban to do so, and the Inland stream by Ria Stars, with both earning promotion to the 2000–01 Premier Soccer League.

Two high-profile clubs were relegated, with Durban Bush Bucks, winners of the inaugural 1985 NSL First Division, finishing bottom of the Coastal Stream, and Vaal Professionals, members of the inaugural 1971 Castle League, finishing bottom of the Inland Stream.