1999–2000 Penn State Lady Lions basketball team

The 1999–2000 Penn State Lady Lions basketball team represented Pennsylvania State University during the 1999–2000 NCAA Division I women's basketball season. The Lady Lions, led by 20th year head coach Rene Portland, played their home games at the Bryce Jordan Center and were members of the Big Ten Conference. They finished the season 30–5 overall, 15–1 in Big Ten play to win the Big Ten Regular Season title. They lost in the championship game to No. 3 seed Purdue in the 2000 Big Ten Conference women's basketball tournament. They were invited to the 2000 NCAA Division I women's basketball tournament where they reached the first Final Four in program history before losing in the National semifinals to eventual champion UConn.

Schedule
!colspan=9 style=| Regular Season

!colspan=9 style= | Big Ten tournament

!colspan=9 style= | NCAA tournament

Source