1999–2000 Tunisian Coupe de la Ligue Professionnelle

The 1999–2000 Tunisian Coupe de la Ligue Professionnelle was the 1st edition of the top knockout tournament for the Tunisian league football clubs.

Round of 16
Espérance Sportive de Tunis, Étoile Sportive du Sahel and Club Africain received a bye to Round of 16. Both Espérance Sportive de Tunis and Étoile Sportive du Sahel withdrew from the competition and were replaced by Jendouba Sport and CO Medenine.