1999–2000 Ukrainian Second League

The 1999–2000 Ukrainian Second League is the ninth season of 3rd level professional football in Ukraine. The competitions are divided into three groups – A, B, and C.

The groups were won respectively by FC Bukovyna Chernivtsi, FC Borysfen Boryspil and FC Dnipro-2 Dnipropetrovsk.

Promoted
The following team were promoted from the 1998–99 Ukrainian Football Amateur League:
 * FC Dynamo Lviv – (debut)
 * FC Arsenal Kharkiv – (debut)
 * FC Obolon-Zmina-2 Kyiv – (debut)

Also, four more clubs were admitted additionally:
 * FC Nyva Vinnytsia – (debut)
 * FC Prykarpattia-2 Ivano-Frankivsk – (debut)
 * FC ADOMS Kremenchuk– (debut)
 * FC Mashynobudivnyk Druzhkivka – (debut)

Relegated
The following team were relegated from the 1998–99 Ukrainian First League:
 * FC Podillya Khmelnytskyi – (returning after an absence of a season)
 * FC Kremin Kremenchuk – (returning after an absence of 8 seasons, previously in the 1991 Soviet tier–3 competitions)
 * FC Bukovyna Chernivtsi – (returning after an absence of 9 seasons, previously in the 1990 Soviet tier–3 competitions)
 * FC Desna Chernihiv – (returning after an absence of 2 seasons)

Group A
New clubs: Bukovyna Chernivtsi, Podillya Khmelnytskyi, Dynamo Lviv, Nyva Vinnytsia (the farm team of FC Vinnytsia), Prykarpattia-2 Ivano-Frankivsk.

Group B
New club: Obolon-Zmina-2 Kyiv

Group C
New clubs: Desna Chernihiv, Kremin Kremenchuk, ADOMS Kremenchuk, Mashynobudivnyk Druzhkivka, Arsenal Kharkiv.