1999 Copa Colsanitas – Singles

The 1999 Copa Colsanitas singles was the singles event of the second edition of the Copa Colsanitas; a WTA Tier IV tournament and the most prestigious women's tennis tournament held in Colombia and Hispanic America. Paola Suárez was the defending champion but lost in the semifinals to Christína Papadáki.

Fabiola Zuluaga won in the final 6–1, 6–3 against Papadáki.

Seeds
• # 🇨🇳 Li Fang (quarterfinals)

• # 🇦🇷 Florencia Labat (first round)

• # 🇺🇸 Meghann Shaughnessy (semifinals)

• # 🇦🇷 Paola Suárez (semifinals)

• # 🇨🇴 Fabiola Zuluaga (champion)

• # Seda Noorlander (second round)

• # 🇦🇷 Mariana Díaz Oliva (second round)

• # 🇩🇪 Jana Kandarr (first round)

Seeds
• # 🇮🇹 Alice Canepa (qualifying competition, lucky loser)

• # 🇦🇺 Jelena Dokić (qualifying competition)

• # 🇺🇸 Tracy Singian (first round)

• # 🇦🇹 Sybille Bammer (first round)

• # 🇨🇦 Martina Nejedly (second round)

• # 🇸🇪 Kristina Triska (second round)

• # 🇦🇷 Luciana Masante (qualifier)

• # 🇦🇷 Celeste Contín (qualifier)

Qualifiers
• # 🇦🇷 Luciana Masante

• # 🇪🇸 Cristina Arribas

• # 🇦🇷 Celeste Contín

• # 🇦🇷 Romina Ottoboni

Lucky losers

 * 1) 🇮🇹 Alice Canepa