1999 Italian Open – Women's singles

Venus Williams defeated Mary Pierce in the final, 6–4, 6–2 to win the women's singles tennis title at the 1999 Italian Open.

Martina Hingis was the defending champion, but lost in the semifinals to Williams in a rematch of the previous year's final.

Seeds
The top eight seeds received a bye to the second round. • # Martina Hingis (semifinals)

• # 🇪🇸 Arantxa Sánchez Vicario (third round)

• # 🇺🇸 Venus Williams (champion)

• # 🇫🇷 Mary Pierce (final)

• # Patty Schnyder (third round)

• # 🇺🇸 Serena Williams (quarterfinals)

• # 🇫🇷 Nathalie Tauziat (third round)

• # 🇫🇷 Sandrine Testud (quarterfinals)

• # 🇷🇺 Anna Kournikova (third round)

• # 🇫🇷 Amélie Mauresmo (semifinals)

• # 🇧🇪 Dominique Van Roost (quarterfinals)

• # Amanda Coetzer (second round)

• # 🇷🇴 Irina Spîrlea (third round)

• # 🇧🇾 Natasha Zvereva (first round)

• # 🇪🇸 Conchita Martínez (third round)

• # 🇷🇺 Elena Likhovtseva (first round)

Seeds
• # n.a.

• # 🇷🇺 Tatiana Panova (qualifying competition, lucky loser)

• # n.a.

• # 🇻🇪 María Vento (qualifier)

• # Christína Papadáki (qualifier)

• # Seda Noorlander (qualifying competition, lucky loser)

• # 🇯🇵 Miho Saeki (first round)

• # 🇧🇪 Sabine Appelmans (qualifier)

• # 🇮🇹 Laura Golarsa (qualifying competition)

• # 🇨🇦 Sonya Jeyaseelan (first round)

Qualifiers
• # 🇮🇹 Germana Di Natale

• # 🇧🇪 Sabine Appelmans

• # 🇻🇪 María Vento

• # 🇮🇹 Francesca Schiavone

• # 🇺🇸 Sandra Cacic

• # 🇷🇺 Elena Dementieva

• # Christína Papadáki

• # 🇮🇹 Antonella Serra Zanetti

Lucky losers

 * 1) 🇷🇺 Tatiana Panova