1999 Nicholls State Colonels football team

The 1999 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Football League during the 1999 NCAA Division I-AA football season. Led by first-year head Daryl Daye, the Colonels compiled an overall record of 1–10 with a mark of 1–6 in conference play, tying for seventh place in the Southland. Nicholls State played home games at John L. Guidry Stadium in Thibodaux, Louisiana.