2000–01 Charlotte 49ers men's basketball team

The 2000–01 UNC Charlotte 49ers men's basketball team represented the University of North Carolina at Charlotte during the 2000–01 college basketball season. This was head coach Bobby Lutz's third season at the school. The 49ers competed in Conference USA and played their home games at Dale F. Halton Arena. They finished the season 22–11 (10–6 in C-USA play) and received an at-large bid to the 2001 NCAA tournament as No. 9 seed in the Midwest region. The 49ers defeated No. 8 seed Tennessee, 70–63, in the opening round before falling to No. 1 seed Illinois in the round of 32.

Schedule and results
!colspan=9 style=| Regular season

!colspan=9 style=| C-USA tournament

!colspan=9 style=| NCAA tournament