2000–01 Cypriot Third Division

The 2000–01 Cypriot Third Division was the 30th season of the Cypriot third-level football league. ASIL Lysi won their 1st title.

Format
Fourteen teams participated in the 2000–01 Cypriot Third Division. All teams played against each other twice, once at their home and once away. The team with the most points at the end of the season crowned champions. The first three teams were promoted to the 2001–02 Cypriot Second Division and the last three teams were relegated to the 2001–02 Cypriot Fourth Division.

Point system
Teams received three points for a win, one point for a draw and zero points for a loss.

Changes from previous season
Teams promoted to 2000–01 Cypriot Second Division
 * THOI Lakatamia
 * Rotsidis Mammari
 * Kinyras Empas

Teams relegated from 1999–2000 Cypriot Second Division
 * PAEEK FC
 * Iraklis Gerolakkou

Teams promoted from 1999–2000 Cypriot Fourth Division
 * MEAP Nisou
 * Elia Lythrodonta
 * THOI Avgorou
 * AMEP Parekklisia

Teams relegated to 2000–01 Cypriot Fourth Division
 * Achyronas Liopetriou
 * Ellinismos Akakiou
 * Doxa Paliometochou