2000–01 Danish 1st Division

The 2000–01 Danish 1st Division season was the 56th season of the Danish 1st Division league championship and the 15th consecutive as a second tier competition governed by the Danish Football Association.

The division-champion and runner-up promoted to the 2001–02 Danish Superliga. The teams in the 14th, 15th and 16th relegated to the 2001–02 Danish 2nd Division.