2000–01 Eerste Divisie

The Dutch Eerste Divisie in the 2000–01 season was contested by 18 teams. FC Den Bosch won the championship.

New entrants
Relegated from the 1999–2000 Eredivisie
 * Cambuur Leeuwarden
 * FC Den Bosch
 * MVV

Promotion/relegation play-offs
In the promotion/relegation competition, eight entrants (six from this league and two from the Eredivisie) entered in two groups. The group winners were promoted to the Eredivisie.