2000–01 Primera B Nacional

The 2000–01 Argentine Primera B Nacional was the 15th season of second division professional of football in Argentina. A total of 30 teams competed; the champion and runner-up were promoted to Argentine Primera División.

Promotion Playoff
The Promotion Playoff was played by the teams placed 1st and 2nd of each zone. The winning team was declared champion and was automatically promoted to Primera División. The teams that lost in semifinal joined into the Quarterfinals of the Second Promotion Playoff, and the team that lost in the final joined in the semifinal of the Second Promotion Playoff.

Semifinals
!colspan="5"|Semifinals

!colspan="5"|Semifinals

1: Qualified because of sport advantage.

Championship final
Note: Banfield won 6–3 on aggregate, promoting to Primera División

Second Promotion Playoff
The Second Promotion Playoff or Torneo Reducido was played by the teams placed 3rd to 6th of each zone. Instituto and Gimnasia y Esgrima (CdU) joined in the Quarterfinals, and Quilmes joined in the Semifinals. The winner was promoted to Primera División.

Bracket
1: Qualified because of sport advantage.
 * Note: The team in the first line plays at home the second leg.

Reducido Final
Note: Nueva Chicago won 4–2 on aggregate, promoting to Primera División.

Promotion Playoff Primera División-Primera B Nacional
The best two teams of each zone that were not promoted (Quilmes and Instituto) played against the 18th and the 17th placed of the Relegation Table of 2000–01 Primera División.

!colspan="5"|Relegation/promotion playoff 1

!colspan="5"|Relegation/promotion playoff 2


 * }
 * Argentinos Juniors remains in Primera División after a 1-1 aggregate tie by virtue of a "sports advantage". In case of a tie in goals, the team from the Primera División gets to stay in it.
 * Belgrano remains in Primera División after a 1-1 aggregate tie by virtue of a "sports advantage". In case of a tie in goals, the team from the Primera División gets to stay in it.
 * Belgrano remains in Primera División after a 1-1 aggregate tie by virtue of a "sports advantage". In case of a tie in goals, the team from the Primera División gets to stay in it.

Relegation
7 teams were relegated: 2 teams with the worst co-efficient from Interior Zone, 2 teams with the worst co-efficient from Metropolitana Zone and 3 more teams regardless their affiliation.

Note: Clubs with indirect affiliation with AFA are relegated to the Torneo Argentino A, while clubs directly affiliated face relegation to Primera B Metropolitana. Clubs with direct affiliation are all from Greater Buenos Aires, with the exception of Newell's, Rosario Central, Central Córdoba and Argentino de Rosario, all from Rosario, and Unión and Colón from Santa Fe.