2000–01 Segunda División B

The 2000–01 Segunda División B season was the 24th edition of the tournament. It started in August 2000 and ended in May 2001.

Summary before the 2000–01 season
Playoffs de Ascenso:


 * Universidad de Las Palmas (P)
 * Ourense
 * Racing de Ferrol (P)
 * Mensajero
 * Gimnástica de Torrelavega
 * Zaragoza B
 * Burgos
 * Barakaldo
 * Gandía
 * Murcia (P)
 * Gramenet
 * Hércules
 * Granada
 * Ceuta
 * Xerez
 * Jaén (P)

Relegated from Segunda División:


 * Atlético Madrid B
 * Toledo
 * Mérida (dissolved)
 * Logroñés (relegated to Tercera División)

Promoted from Tercera División:


 * Deportivo La Coruña B (from Group 1)
 * Siero (from Group 2)
 * AD Universidad de Oviedo (from Group 2)
 * Tropezón (from Group 3)
 * Racing de Santander B (from Group 3)
 * Eibar B (from Group 4)
 * Espanyol B (from Group 5)
 * Mataró (from Group 5)
 * Burriana (from Group 6)
 * Benidorm (from Group 6)
 * Alcorcón (from Group 7)
 * Ejido (from Group 9)
 * Linares (from Group 9)
 * Almería (from Group 9)
 * Algeciras (from Group 10)
 * Vecindario (from Group 12)
 * Peña Sport (from Group 15)

Relegated:


 * Lanzarote
 * Oviedo B
 * Gimnástica Segoviana
 * Móstoles
 * Bermeo
 * Valladolid B
 * Figueruelas
 * Izarra
 * Valencia B
 * Yeclano
 * Ontinyent
 * Lorca
 * Betis B
 * Cacereño
 * Sevilla B
 * Águilas
 * Avilés
 * Fuenlabrada

Dissolved:
 * Manchego

Occupied the vacant spots by administrative relegations:
 * Chantrea (occupied the vacant spot of Logroñés)

Occupied the vacant spots by clubs dissolutions:
 * San Fernando (occupied the vacant spot of Manchego)
 * Don Benito (occupied the vacant spot of Mérida)

Group I
Teams from Asturias, Canary Islands, Castile and León, Castilla–La Mancha, Community of Madrid and Galicia.

Group II
Teams from Aragon, Basque Country, Cantabria, Castile and León, La Rioja and Navarre.

Group III
Teams from Balearic Islands, Castilla–La Mancha, Catalonia, Region of Murcia and Valencian Community.

Group IV
Teams from Andalusia, Castilla–La Mancha, Ceuta, Extremadura and Melilla.

League Table
1. Polideportivo Almería withdrew after 18 games.