2000 Betty Barclay Cup – Singles

Venus Williams was the defending champion, but lost in quarterfinals to Amanda Coetzer.

Martina Hingis won the title by defeating Arantxa Sánchez Vicario 6–3, 6–3 in the final.

Seeds
The first four seeds received a bye into the second round. • # Martina Hingis (champion)

• # 🇺🇸 Venus Williams (quarterfinals)

• # 🇪🇸 Conchita Martínez (quarterfinals)

• # 🇪🇸 Arantxa Sánchez Vicario (final)

• # 🇦🇹 Barbara Schett (first round, retired)

• # 🇷🇺 Anna Kournikova (quarterfinals)

• # 🇩🇪 Anke Huber (semifinals)

• # Amanda Coetzer (semifinals)