2000 Copa Colsanitas – Singles

Fabiola Zuluaga was the defending champion, but lost in second round to runner-up Tathiana Garbin.

Patricia Wartusch won the title by defeating Tathiana Garbin 4–6, 6–1, 6–4 in the final.

Seeds
• # 🇦🇹 Sylvia Plischke (semifinals)

• # 🇨🇴 Fabiola Zuluaga (second round)

• # 🇪🇸 Cristina Torrens Valero (first round)

• # 🇪🇸 Ángeles Montolio (second round)

• # Emmanuelle Gagliardi (first round)

• # 🇦🇷 Paola Suárez (quarterfinals)

• # 🇧🇾 Olga Barabanschikova (quarterfinals)

• # 🇮🇹 Rita Grande (first round)