2000 Croatian Bol Ladies Open – Singles

Corina Morariu was the defending champion, but lost in the quarterfinals to Amélie Mauresmo.

Tina Pisnik won the title by defeating Mauresmo 7–6(7–4), 7–6(7–2) in the final.

Seeds
The first two seeds received a bye to the second round. • # 🇫🇷 Julie Halard-Decugis (second round)

• # 🇫🇷 Sandrine Testud (quarterfinals)

• # 🇫🇷 Amélie Mauresmo (final)

• # Silvija Talaja (second round)

• # 🇺🇸 Corina Morariu (quarterfinals)

• # 🇮🇹 Silvia Farina (first round)

• # 🇦🇷 Paola Suárez (quarterfinals)

• # Cara Black (second round)