2000 Estoril Open – Women's singles

Katarina Srebotnik was the defending champion, but lost in first round to Seda Noorlander.

Anke Huber won the title by defeating Nathalie Dechy 6–2, 1–6, 7–5 in the final.

Seeds
• # 🇩🇪 Anke Huber (champion)

• # 🇫🇷 Nathalie Dechy (final)

• # 🇫🇷 Anne-Gaëlle Sidot (second round)

• # 🇫🇷 Sarah Pitkowski (first round)

• # 🇮🇹 Silvia Farina (semifinals)

• # 🇪🇸 Cristina Torrens Valero (quarterfinals)

• # 🇭🇺 Rita Kuti-Kis (quarterfinals)

• # 🇪🇸 Ángeles Montolio (quarterfinals)