2000 Internazionali Femminili di Palermo – Singles

Anastasia Myskina was the defending champion, but lost in first round to Justine Henin.

Henrieta Nagyová won the title by defeating Pavlina Nola 6–3, 7–5 in the final.

Seeds
• # Silvija Talaja (first round, retired)

• # Ruxandra Dragomir (second round)

• # 🇷🇺 Anastasia Myskina (first round)

• # 🇮🇹 Tathiana Garbin (first round)

• # 🇮🇹 Silvia Farina (semifinals)

• # 🇮🇱 Anna Smashnova (semifinals)

• # 🇮🇹 Rita Grande (first round)

• # 🇪🇸 Marta Marrero (first round)