2000 NCAA Division I baseball tournament

The 2000 NCAA Division I baseball tournament was played at the end of the 2000 NCAA Division I baseball season to determine the national champion of college baseball. The tournament concluded with eight teams competing in the College World Series, a double-elimination tournament in its fifty fourth year. Sixteen regional competitions were held to determine the participants in the final event, with each winner advancing to a best of three series against another regional champion for the right to play in the College World Series. Each region was composed of four teams, resulting in 64 teams participating in the tournament at the conclusion of their regular season, and in some cases, after a conference tournament. The fifty-fourth tournament's champion was LSU, coached by Skip Bertman. The Most Outstanding Player was Trey Hodges of LSU.

National seeds
Bold indicates CWS participant.
 * 1) South Carolina
 * 2) LSU
 * 3) Georgia Tech
 * 4) Clemson
 * 5) Houston
 * 6) Florida State
 * 7) Arizona State
 * 8) Stanford

Regionals and super regionals
Bold indicates winner. * indicates host.

Austin Super Regional
Hosted by Texas at Disch–Falk Field in Austin, Texas

Notes on tournament field

 * Army, Butler, and Wagner were making their first NCAA tournament appearance.

All-Tournament Team
The following players were members of the College World Series All-Tournament Team.

Notable players

 * Clemson:
 * Florida State: Marshall McDougall
 * LSU: Trey Hodges, Brian Tallet, Ryan Theriot, Mike Fontenot, Brad Hawpe, Brad Cresse, Ray Wright
 * Louisiana–Lafayette: Scott Dohmann
 * San Jose State:
 * Stanford: Joe Borchard, Justin Wayne
 * Texas: Beau Hale, Phil Seibel, Ryan Hubele, Tommy Nicholson, Todd West, Charlie Thames
 * USC: Mark Prior, Anthony Reyes