2000 NCAA Division I men's soccer tournament

The 2000 NCAA Division I men's soccer tournament was the 41st organized men's college soccer tournament by the National Collegiate Athletic Association, to determine the top college soccer team in the United States. The Connecticut Huskies won their second national title by defeating the Creighton Bluejays in the championship game, 2–0. The semifinals and final were played in Charlotte, North Carolina at Ericsson Stadium for the second straight year. All other games were played at the home field of the higher seeded team.