2000 NCAA Division I women's lacrosse tournament

The 2000 NCAA Division I Women's Lacrosse Championship was the 19th annual single-elimination tournament to determine the national champion of Division I NCAA women's college lacrosse. The championship game was played at Lions Stadium in Trenton, New Jersey during May 2000. All NCAA Division I women's lacrosse programs were eligible for this championship. Ultimately, 12 teams were invited to the tournament.

Maryland defeated Princeton, 16–8, to win their eighth overall and sixth consecutive, national championship. This would subsequently become the sixth of Maryland's record seven straight national titles (1995–2001).

For the second consecutive year, the leading scorer for the tournament was Jen Adams from Maryland, with 22 goals. Adams was also again named the tournament's Most Outstanding Player.

All-tournament team

 * Alivian Coates, James Madison
 * Kristin Hagart, Loyola (MD)
 * Jess Marion, James Madison
 * Stacey Moriand, Loyola (MD)
 * Jen Adams, Maryland (Most outstanding player)
 * Christie Jenkins, Maryland
 * Alex Kahoe, Maryland
 * Tonia Porras, Maryland
 * Brooke Owens, Princeton
 * Julie Shaner, Princeton
 * Lauren Simone, Princeton