2000 Pilot Pen Tennis – Singles

Venus Williams was the defending champion and successfully defended her title by defeating Monica Seles 6–2, 6–4 in the final.

Seeds
The first four seeds received a bye into the second round. • # 🇺🇸 Venus Williams (champion)

• # 🇺🇸 Monica Seles (final)

• # 🇫🇷 Nathalie Tauziat (semifinals)

• # 🇩🇪 Anke Huber (quarterfinals)

• # Amanda Coetzer (semifinals)

• # 🇧🇪 Dominique van Roost (quarterfinals)

• # 🇦🇹 Barbara Schett (first round)

• # 🇫🇷 Julie Halard-Decugis (first round)