2000 Prairie View A&M Panthers football team

The 2000 Prairie View A&M Panthers football team represented Prairie View A&M University as a member of the Southwestern Athletic Conference (SWAC) during the 2000 NCAA Division I-AA football season. Led by first-year head coach Larry Dorsey, the Panthers compiled an overall record of 2–9, with a mark of 2–5 in conference play, and finished fifth in the SWAC West Division.