2000 Sanex Trophy – Singles

María Sánchez Lorenzo was the defending champion, but did not compete this year.

Anna Smashnova won the title by defeating Dominique Van Roost 6–2, 7–5 in the final.

Seeds
• # 🇧🇪 Dominique Van Roost (final)

• # 🇧🇪 Sabine Appelmans (quarterfinals)

• # 🇪🇸 Magüi Serna (semifinals)

• # 🇫🇷 Sarah Pitkowski (quarterfinals)

• # 🇭🇺 Rita Kuti-Kis (first round)

• # 🇮🇱 Anna Smashnova (champion)

• # 🇧🇾 Natasha Zvereva (withdrew)

• # 🇪🇸 Marta Marrero (semifinals)

• # 🇧🇾 Olga Barabanschikova (first round)