2000 Toyota Princess Cup – Singles

Lindsay Davenport was the defending champion, but did not compete this year.

Serena Williams won the title by defeating Julie Halard-Decugis 7–5, 6–1 in the final.

Seeds
The first four seeds received a bye into the second round. • # 🇺🇸 Monica Seles (semifinals, retired)

• # 🇺🇸 Serena Williams (champion)

• # 🇫🇷 Amélie Mauresmo (quarterfinals)

• # 🇫🇷 Julie Halard-Decugis (final)

• # 🇺🇸 Amy Frazier (quarterfinals)

• # 🇺🇸 Kristina Brandi (quarterfinals)

• # 🇯🇵 Ai Sugiyama (second round)

• # 🇦🇺 Jelena Dokic (quarterfinals)