2000 WTA Madrid Open – Singles

Lindsay Davenport was the reigning champion, but did not compete this year.

Gala León García won the title by defeating Fabiola Zuluaga 4–6, 6–2, 6–2 in the final. It was the 1st and only title in her career.

Seeds
The first two seeds received a bye into the second round. • # 🇫🇷 Mary Pierce (second round)

• # 🇺🇸 Amy Frazier (second round)

• # 🇯🇵 Ai Sugiyama (second round)

• # 🇺🇸 Lisa Raymond (first round)

• # 🇦🇹 Sylvia Plischke (first round)

• # 🇦🇷 Paola Suárez (second round)

• # 🇺🇸 Kristina Brandi (second round)

• # 🇪🇸 Gala León García (champion)