2000 du Maurier Open – Women's singles

Defending champion Martina Hingis won the women's singles tennis title at the 2000 Canadian Open after Serena Williams retired from the final due to a left foot injury, with the score at 0–6, 6–3, 3–0.

Seeds
The top eight seeds received a bye into the second round.


 * 1)  Martina Hingis (champion)
 * 2) 🇺🇸 Lindsay Davenport (third round, retired due to a left ankle injury)
 * 3) 🇪🇸 Conchita Martínez (semifinals)
 * 4) 🇺🇸 Serena Williams (final, retired due to a left foot injury)
 * 5) 🇫🇷 Nathalie Tauziat (second round)
 * 6) 🇩🇪 Anke Huber (third round)
 * 7) 🇪🇸 Arantxa Sánchez Vicario (semifinals)
 * 8) 🇫🇷 Sandrine Testud (quarterfinals)
 * 9)  Amanda Coetzer (second round)
 * 10) 🇫🇷 Julie Halard-Decugis (third round, withdrew due to lower back pain)
 * 11) 🇷🇺 Anna Kournikova (third round)
 * 12) 🇧🇪 Dominique Van Roost (first round)
 * 13) 🇺🇸 Jennifer Capriati (third round)
 * 14) 🇦🇹 Barbara Schett (first round)
 * 15) 🇺🇸 Chanda Rubin (third round)
 * 16) 🇺🇸 Amy Frazier (quarterfinals)