2001 Estoril Open – Women's singles

Anke Huber was the defending champion but lost in the first round to Denisa Chládková.

Ángeles Montolio won in the final 3–6, 6–3, 6–2 against Elena Bovina.

Seeds
A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated.


 * 1) 🇩🇪 Anke Huber (first round)
 * 2) 🇧🇬 Magdalena Maleeva (second round)
 * 3) 🇧🇪 Justine Henin (semifinals)
 * 4) 🇦🇹 Barbara Schett (second round)
 * 5) 🇪🇸 Magüi Serna (second round)
 * 6) 🇱🇺 Anne Kremer (second round)
 * 7)  Silvija Talaja (first round)
 * 8) 🇮🇹 Tathiana Garbin (quarterfinals)