2001 Italian Open – Women's singles

Jelena Dokic defeated Amélie Mauresmo in the final, 7–6(7–3), 6–1 to win the women's singles tennis title at the 2001 Italian Open.

Monica Seles was the reigning champion, but did not compete this year.

Seeds
• # Martina Hingis (semifinals)

• # 🇺🇸 Jennifer Capriati (second round)

• # 🇪🇸 Conchita Martínez (semifinals)

• # 🇫🇷 Amélie Mauresmo (final)

• # 🇫🇷 Nathalie Tauziat (second round)

• # 🇧🇪 Kim Clijsters (second round)

• # 🇪🇸 Arantxa Sánchez Vicario (quarterfinals)

• # 🇧🇬 Magdalena Maleeva (second round)

• # 🇩🇪 Anke Huber (first round)

• # 🇫🇷 Mary Pierce (first round)

• # 🇧🇪 Justine Henin (withdrew)

• # 🇫🇷 Sandrine Testud (first round)

• # 🇺🇸 Meghann Shaughnessy (second round, withdrew)

• # Jelena Dokic (champion)

• # 🇦🇹 Barbara Schett (first round)

• # 🇪🇸 Magüi Serna (first round)

• # 🇦🇷 Paola Suárez (quarterfinals)