2001 NCAA Division III women's basketball tournament

The 2001 NCAA Division III women's basketball tournament was the 20th annual tournament hosted by the NCAA to determine the national champion of Division III women's collegiate basketball in the United States.

Three-time defending champions Washington University in St. Louis defeated Messiah University in the championship game, 67–45, to claim the Bears' fourth Division III national title, their fourth of four consecutive.

The championship rounds were hosted at the O'Neill Center at Western Connecticut State University in Danbury, Connecticut from March 16–17, 2001.

Bracket

 * An asterisk by a team indicates the host of first and second round games
 * An asterisk by a score indicates an overtime period

All-tournament team

 * Tasha Rodgers, Washington University in St. Louis
 * Jennifer Rudis, Washington University in St. Louis
 * Katy Sturtz, Ohio Wesleyan
 * Amy Hitz, Messiah
 * Brianne Bognanno, Emmanuel (MA)