2005 Copa Colsanitas Seguros Bolívar – Singles

Four-time champion Fabiola Zuluaga failed to defend her title after losing to Lourdes Domínguez Lino in the semifinals. The loss also ends Zuluaga's 17-match winning streak at her home soil.

Flavia Pennetta won the title by defeating Lourdes Domínguez Lino 7–6(7–4), 6–4 in the final.

Seeds
• # 🇨🇴 Fabiola Zuluaga (semifinals)

• # 🇮🇹 Flavia Pennetta (champions)

• # 🇫🇷 Émilie Loit (second round)

• # 🇪🇸 María Sánchez Lorenzo (second round)

• # 🇨🇿 Barbora Strýcová (quarterfinals)

• # 🇪🇸 Arantxa Parra Santonja (first round)

• # 🇺🇦 Tatiana Perebiynis (first round)

• # 🇸🇰 Ľubomíra Kurhajcová (second round)