2005 Estoril Open – Women's singles

Émilie Loit was the defending champion, but did not compete this year.

Lucie Šafářová won the title by defeating Li Na 6–7(4–7), 6–4, 6–3 in the final.

Seeds
• # 🇮🇹 Flavia Pennetta (first round)

• # 🇷🇺 Dinara Safina (semifinals)

• # 🇦🇷 Gisela Dulko (semifinals)

• # 🇨🇳 Li Na (final)

• # 🇪🇸 Anabel Medina Garrigues (withdrew due to a right knee tendonitis)

• # Dally Randriantefy (quarterfinals)

• # 🇪🇸 Nuria Llagostera Vives (withdrew due to an upper right leg injury)

• # 🇪🇸 Virginia Ruano Pascual (first round)

• # 🇨🇳 Zheng Jie (quarterfinals)

• # 🇪🇸 Arantxa Parra Santonja (first round)