2005 Italian Open – Women's singles

Defending champion Amélie Mauresmo defeated Patty Schnyder in the final, 2–6, 6–3, 6–4 to win the women's singles tennis title at the 2005 Italian Open.

Seeds
The first eight seeds received a bye into the second round. • # 🇷🇺 Maria Sharapova (semifinals)

• # 🇫🇷 Amélie Mauresmo (champion)

• # 🇺🇸 Serena Williams (second round)

• # 🇷🇺 Elena Dementieva (second round)

• # 🇷🇺 Svetlana Kuznetsova (second round)

• # 🇷🇺 Vera Zvonareva (semifinals)

• # 🇷🇺 Nadia Petrova (third round, withdrew due to a right thigh strain)

• # Patty Schnyder (final)

• # 🇷🇺 Elena Bovina (quarterfinals)

• # 🇫🇷 Nathalie Dechy (first round)

• # 🇷🇺 Elena Likhovtseva (first round)

• # 🇫🇷 Tatiana Golovin (first round)

• # 🇮🇹 Silvia Farina Elia (third round)

• # 🇯🇵 Shinobu Asagoe (first round)

• # 🇯🇵 Ai Sugiyama (third round)

• # 🇸🇰 Daniela Hantuchová (first round)